In scientific research and medical advancements, the preservation of biological material through cryopreservation technique provides the foundation for future advancements. Using cryovials which are made specifically for this purpose ensures that the valuable resources remain intact and usable. In this comprehensive guide, we will discuss the best practices for safeguarding biological material through cryopreservation and the critical role of cryovials in this process.
Why Preserve Biological Materials at Ultra Low Temperature?
Preservation at ultralow temperatures, as low as -196°C, is imperative for maintaining the viability and functionality of biological materials over extended periods. By arresting all physical, chemical, and biological processes, cryopreservation ensures that cells, tissues, and organoids remain intact for future use. The cooling should be done at a controlled temperature to avoid the formation of ice crystals, if cells are cooled too quickly it can damage the cell membrane leading to cell death. Cryopreservation prevents the cell from death and it can also be referred to as cells in a hibernating stage.
All cryogenic liquids are gases at normal temperatures, they must be cooled below room temperature before an increase in pressure can liquefy them upon removal from low-temperature storage. Liquid nitrogen that may have seeped into the closed tube may suddenly expand (690 times) during conversion from gas to the liquid phase. When not handled properly, the cryovials may explode and result in physical injuries and exposure to the vial contents. Hence it is to be noted to cryopreserve in the vapour phase of liquid nitrogen.
This preservation method acts as a vital resource for various fields, including regenerative medicine, biobanking, and genetic research, facilitating advancements in understanding diseases and developing therapeutic interventions.
Choose the Right Cryovials
When selecting cryovials, it’s crucial to consider various factors to ensure the integrity and viability of your valuable resources. Here are key features to look for:
- Temperature Stability: Opt for cryovials with temperature stability of up to -196°C (in vapour-phase liquid nitrogen) to safeguard your samples during long-term storage.
- Seal Security: Look for quick seal closure and leak-proof tube seals to prevent any potential sample contamination or loss.
- Contamination Prevention: Choose cryovials that are externally threaded without an O-ring, minimizing the risk of contamination.
- Sample Recovery: V-shaped bottoms allow for maximum sample recovery, ensuring that no sample is wasted.
- Autoclavable: Ensure your cryovials are autoclavable at 121°C for 15 minutes, facilitating sterilization for reuse.
- Stability and Efficiency: Cryovials with hepta feet offer better stability and self-standing capability, optimizing storage efficiency.
- Easy Identification: Seek cryovials with large, clear patches for personalized labeling, facilitating easy sample identification.
- Certification: Confirm that the cryovials are certified DNase, RNase, and endotoxin-free, ensuring the purity of your samples.
How to Prevent Cryo Damage
- Use of Cryoprotectants: Incorporating cryoprotectants such as glycerol, ethylene glycol, or dimethyl sulfoxide (DMSO) is essential to prevent ice crystallization and cellular damage during freezing. DMSO and glycerol are generally used in concentrations ranging from 5-10% and are not used together. Optimal concentrations vary depending on cell type, with careful consideration given to the most tolerant concentration.
- Slow Freezing: Gradual cooling mitigates water loss from cells, minimizing internal ice formation and preserving cellular integrity. The rate of cooling should be tailored to the permeability of the cells being preserved, ensuring optimal results without compromising viability.
Application of Cryopreservation
Cryopreservation finds extensive application in various domains, including:
- Biobanking: Preserving biological specimens for research and clinical purposes.
- Assisted Reproductive Technologies (ART): Storing gametes and embryos for fertility treatments.
- Tissue Engineering: Maintaining tissue constructs for regenerative medicine applications.
Accumax Cryovials – Your Trusted Partner in Cryopreservation
At Accumax, we recognize the significance of reliable cryovials in preserving biological material with utmost safety and efficiency. Our range of cryovials, spanning volumes from 1ml to 5ml, offers unparalleled flexibility in storage requirements. Featuring an externally threaded design for easy handling and a sterility assurance level of Sal 10-6, our cryovials ensure minimal contamination risk, safeguarding the integrity of your valuable specimens.
In summary, adopting best practices in cryopreservation, coupled with the utilization of high-quality cryovials, is crucial in safeguarding biological material and advancing scientific knowledge and innovation.
Aanak Goswami
12+ Years of experience in generating growth for organisations. Having customer management skills with experience of Research, Institutional, clinical diagnostics, Healthcare and Biopharma customers.
