Accurate pipetting is essential for reliable lab work, and maintaining consistency can be difficult when issues arise. At Accumax, we recognize the significance of precise liquid handling for achieving the best results. Here’s a guide to help you troubleshoot and resolve some common pipetting problems.
1. Inconsistent Volume Dispensing
- Cause: This issue often arises from improper calibration or pipetting technique.
- Solution: Make it a habit to regularly calibrate your micropipettes and practice smooth, consistent plunger operation. Avoid pressing or releasing the plunger too quickly, as this can lead to inconsistencies.
2. Dripping or Leaking Tips
- Cause: A poor tip fit or damaged internal seals are common causes.
- Solution: Always use high-quality tips that fit securely on the pipette. Regularly check O-rings and seals, replacing them as needed to ensure a proper seal.
3. Air Bubbles in the Sample
- Cause: Incorrect aspiration techniques, such as tilting the pipette or aspirating too quickly, can introduce air bubbles.
- Solution: Keep the pipette vertical while aspirating, and pull the liquid up at a controlled, steady speed. Pre-wetting the tip before aspiration can also help improve volume consistency.
4. Sticky Plunger Movement
- Cause: This can happen due to residue buildup or mechanical wear over time.
- Solution: Clean the piston regularly and lubricate it according to the manufacturer’s guidelines. If the issue persists, consider having the pipette serviced to prevent further wear or damage.
5. Incorrect Volume Delivery
- Cause: User error or damage to the pipette can lead to inaccurate volume delivery.
- Solution: Always pre-wet the pipette tip for viscous liquids, maintain a consistent speed while pipetting, and visually inspect the pipette mechanism for any signs of damage.
Best Practices for Reliable Pipetting
- Regular Maintenance: Make it a habit to clean and calibrate your pipettes regularly.
- Proper Tip Usage: Always check that the tips you use are compatible and of good quality to ensure a proper seal.
- Consistent Technique: Develop a steady hand and apply uniform pressure to minimize errors.
Precision in pipetting is essential in any laboratory. At Accumax, we are dedicated to enhancing your workflow with dependable equipment and support. We are a renowned global manufacturer of laboratory liquid handling instruments since last 2 decades. By following these guidelines, you can troubleshoot effectively and uphold the high standards your lab requires.
Aanak Goswami
Designation: Global Marketing Head.
With over 13 years of experience in driving organizational growth through strategic business development and sales initiatives, Aanak, specializes in the Research, Institutional, Clinical Diagnostics, Healthcare, and Biopharma sectors. A key aspect of his expertise is leveraging industry insights to generate impactful growth and develop lasting business relationships.
